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验证数据展示

  • WB analysis of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

    WB analysis of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 12896-1-AP (same clone as 12896-1-PBS)

    IP experiment of mouse testis using 12896-1-AP (same clone as 12896-1-PBS)

    IP result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

    IF Staining of HeLa using 12896-1-AP (same clone as 12896-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12896-1-PBS in a different storage buffer formulation.

DTL Polyclonal antibody, PBS Only

DTL Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12896-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CDT2, CDW1, DCAF2, DDB1- and CUL4-associated factor 2, Denticleless protein homolog

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® DTL Ready-To-Use IHC Kit. DTL primary antibody included.

产品信息

12896-1-PBS targets DTL in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3589

Product name: Recombinant human DTL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC033540

Sequence: MLFNSVLRQPQLGVLRNGWSSQYPLQSLLTGYQCSGNDEHTSYGETGVPVPPFGCTFSSAPNMEHVLAVANEEGFVRLYNTESQSFRKKCFKEWMAHWNAVFDLAWVPGELKLVTAAGDQTAKFWDVKAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTSDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKVWDLRKNYTAYRQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAIFNGHQNSTFYVKSSLSPDDQFLVSGSSDEAAYIWKVST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 denticleless homolog (Drosophila)
别名 CDT2, CDW1, DCAF2, DDB1- and CUL4-associated factor 2, Denticleless protein homolog
计算分子量 730 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC033540
基因名称 DTL
Gene ID (NCBI) 51514
RRIDAB_10639514
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZJ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

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