验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20102-1-AP targets DSTYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | DSTYK fusion protein Ag13678 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual serine/threonine and tyrosine protein kinase |
| 别名 | RIPK5, Receptor-interacting serine/threonine-protein kinase 5, KIAA0472, HDCMD38P, EC:2.7.12.1 |
| 计算分子量 | 66 kDa, 105 kDa |
| 观测分子量 | 66 kDa, 100~105 kDa |
| GenBank蛋白编号 | BC060870 |
| 基因名称 | DSTYK |
| Gene ID (NCBI) | 25778 |
| RRID | AB_10694146 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6XUX3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DSTYK (Dual serine-threonine and tyrosine protein kinase gene), also known as CAKUT1, DustyPK, SPG23 has been classically considered as RIPK5, encodes a protein product consisting of 850-931 amino acids, which has a striking membrane-associated distribution in mesenchymal-derived cells of all major organs including brain, heart, kidney, lung, colon, and muscle (PMID: 34853310). DSTYK is strongly expressed in the cytoplasm of most malignant cells and that the high expression of DSTYK in human colon cancer specimens is associated with tumor progression and/or poor prognosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DSTYK antibody 20102-1-AP | Download protocol |
| IF protocol for DSTYK antibody 20102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. | ||
Cell Death Dis Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma
| ||