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  • Featured Product
  • KD/KO Validated

RIPK5 Polyclonal antibody

RIPK5 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20102-1-AP

Print datasheet

Synonyms

DSTYK, Dusty PK, Dusty protein kinase, DustyPK, HDCMD38P, KIAA0472, RIP homologous kinase, RIP5, RIPK5, SgK496, Sugen kinase 496

验证数据展示

  • WB analysis using 20102-1-AP

    WB analysis using 20102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20102-1-AP (RIPK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20102-1-AP

    IF Staining of HepG2 using 20102-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIPK5 antibody (20102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat liver tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20102-1-AP is RIPK5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Immunogen RIPK5 fusion protein Ag13678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual serine/threonine and tyrosine protein kinase
Synonyms DSTYK, Dusty PK, Dusty protein kinase, DustyPK, HDCMD38P, KIAA0472, RIP homologous kinase, RIP5, RIPK5, SgK496, Sugen kinase 496
Calculated Molecular Weight 66 kDa, 105 kDa
GenBank Accession NumberBC060870
Gene Symbol DSTYK
Gene ID (NCBI) 25778
RRIDAB_10694146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6XUX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein-serine/threonine and protein-tyrosine kinases (PSK and PTK) comprise two major classes of protein kinases. They provide a key switch dictating the dynamic nature and graded regulation of signal transduction pathway activities in cellular organization. DSTYK is a members of the RIP serine/threonine kinase family which involved in activation of NF-KB, JNK, and p38, and induction of apoptosis. This antibody is specific to DSTYK.

实验方案

Product Specific Protocols
WB protocol for RIPK5 antibody 20102-1-APDownload protocol
IF protocol for RIPK5 antibody 20102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyIF

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun
View Article
human,mouseWB,IHC

Cell Death Dis

Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma

Authors - Chenxi Zhong
  • KD Validated
View Article