验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, LNCaP cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
55396-1-AP targets DSPP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dentin sialophosphoprotein |
| 别名 | Dentin phosphophoryn, dentin sialophosphoprotein, DFNA39, DGI1, DMP3, DPP, DPP Dentin sialoprotein, DSP, DSPP, DTDP2 |
| 计算分子量 | 131 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | NM_014208 |
| 基因名称 | DSPP |
| Gene ID (NCBI) | 1834 |
| RRID | AB_3086448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZW4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DSPP antibody 55396-1-AP | Download protocol |
| WB protocol for DSPP antibody 55396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo | ||
Arch Oral Biol NOTUM plays a bidirectionally modulatory role in the odontoblastic differentiation of human stem cells from the apical papilla through the WNT/β-catenin signaling pathway | ||
Eur J Pharmacol Vdr mediates Wnt signaling pathway to regulate odontoblasts differentiation during dentin apposition | ||
Int Endod J Effects of phase-transited lysozyme on adhesion, migration and odontogenic differentiation of human dental pulp cells: An in vitro study |
