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验证数据展示

  • WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

    WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

    IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

DSP Monoclonal antibody, PBS Only

DSP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68364-1-PBS

产品说明书

CloneNo. 1B10D4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

desmoplakin, DP, DPI, DPII, DSP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68364-1-PBS targets DSP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag30499

Product name: Recombinant human DSP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC140802

Sequence: MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 desmoplakin
别名 desmoplakin, DP, DPI, DPII, DSP
计算分子量 2871 aa, 332 kDa
观测分子量 240-330 kDa
GenBank蛋白编号BC140802
基因名称 DSP
Gene ID (NCBI) 1832
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP15924
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

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