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验证数据展示

  • WB analysis using 29942-1-AP (same clone as 29942-1-PBS)

    WB analysis using 29942-1-AP (same clone as 29942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29942-1-AP (DSG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung squamous cell carcinoma using 29942-1-AP (same clone as 29942-1-PBS)

    IHC staining of human lung squamous cell carcinoma using 29942-1-AP (same clone as 29942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

DSG3 Polyclonal antibody, PBS Only

DSG3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29942-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PVA, DSG 3, Desmoglein-3, Desmoglein 3, CDHF6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29942-1-PBS targets DSG3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30880

Product name: Recombinant human DSG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 760-880 aa of NM_001944

Sequence: SGQSGTMRTRHSTGGTNKDYADGAISMNFLDSYFSQKAFACAEEDDGQEANDCLLIYDNEGADATGSPVGSVGCCSFIADDLDDSFLDSLGPKFKKLAEISLGVDGEGKEVQPPSKDSGYG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 desmoglein 3 (pemphigus vulgaris antigen)
别名 PVA, DSG 3, Desmoglein-3, Desmoglein 3, CDHF6
计算分子量 108 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001944
基因名称 DSG3
Gene ID (NCBI) 1830
RRIDAB_3086196
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32926
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DSG3, Desmoglein-3, is the component of intercellular desmosome junctions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG3 is expressed in the basal lower layers of in the skin epidermis (PMID: 30528827). It acts as a prognostic marker of Esophageal Squamous cell carcinoma (PMID:24568510).

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