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验证数据展示

  • WB analysis using 87426-1-RR

    WB analysis using 87426-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87426-1-RR (DSG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 87426-1-RR

    IF Staining of A431 using 87426-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 87426-1-RR

    IF Staining of MCF-7 using 87426-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87426-1-RR

    Affinity of 87426-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87426-1-RR against Human DSG2 were performed. The affinity constant is 2.32 nM.

DSG2 Recombinant monoclonal antibody

DSG2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87426-1-RR

产品说明书

CloneNo. 251405G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, A431 cells
Positive IF/ICC detected inA431 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy DSG2 Ready-To-Use IHC Kit. DSG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87426-1-RR targets DSG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6509

Product name: Recombinant human DSG2 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 50-609 aa of NM_001943.4

Sequence: AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 desmoglein 2
别名 ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2
计算分子量122 kDa
观测分子量150 kDa
GenBank蛋白编号NM_001943.4
基因名称 DSG2
Gene ID (NCBI) 1829
RRIDAB_3745538
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14126
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

实验方案

Product Specific Protocols
IF protocol for DSG2 antibody 87426-1-RRDownload protocol
WB protocol for DSG2 antibody 87426-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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