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验证数据展示

  • WB analysis of COLO 320 using 60041-1-Ig (same clone as 60041-1-PBS)

    WB analysis of COLO 320 using 60041-1-Ig (same clone as 60041-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60041-1-Ig (DSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DSE antibody included
  • IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DSE antibody included
  • IF Staining of HeLa using 60041-1-Ig (same clone as 60041-1-PBS)

    IF Staining of HeLa using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSE antibody (60041-1-Ig, Clone: 2C9F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

DSE Monoclonal antibody, PBS Only

DSE Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60041-1-PBS

产品说明书

CloneNo. 2C9F7

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DSE Ready-To-Use IHC Kit. DSE primary antibody included.

产品信息

60041-1-PBS targets DSE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0695

Product name: Recombinant human DSE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 659-958 aa of BC039245

Sequence: RAAYLFIGPSIDVQSFTVHGDSQQLDVFIATSKHAYATYLWTGEATGQSAFAQVIADRHKILFDRNSAIKSSIVPEVKDYAAIVEQNLQHFKPVFQLLEKQILSRVRNTASFRKTAERLLRFSDKRQTEEAIDRIFAISQQQQQQSKSKKNRRAGKRYKFVDAVPDIFAQIEVNEKKIRQKAQILAQKELPIDEDEEMKDLLDFADVTYEKHKNGGLIKGRFGQARMVTTTHSRAPSLSASYTRLFLILNIAIFFVMLAMQLTYFQRAQSLHGQRCLYAVLLIDSCILLWLYSSCSQSQC

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 dermatan sulfate epimerase
别名 dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2
计算分子量 958 aa, 110 kDa
观测分子量 100 kDa
GenBank蛋白编号BC039245
基因名称 DSE
Gene ID (NCBI) 29940
RRIDAB_2093286
偶联类型 Unconjugated
形式Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ9UL01
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

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