验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60041-1-Ig targets DSE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag0695 Product name: Recombinant human DSE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 659-958 aa of BC039245 Sequence: RAAYLFIGPSIDVQSFTVHGDSQQLDVFIATSKHAYATYLWTGEATGQSAFAQVIADRHKILFDRNSAIKSSIVPEVKDYAAIVEQNLQHFKPVFQLLEKQILSRVRNTASFRKTAERLLRFSDKRQTEEAIDRIFAISQQQQQQSKSKKNRRAGKRYKFVDAVPDIFAQIEVNEKKIRQKAQILAQKELPIDEDEEMKDLLDFADVTYEKHKNGGLIKGRFGQARMVTTTHSRAPSLSASYTRLFLILNIAIFFVMLAMQLTYFQRAQSLHGQRCLYAVLLIDSCILLWLYSSCSQSQC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dermatan sulfate epimerase |
| 别名 | dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2 |
| 计算分子量 | 958 aa, 110 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC039245 |
| 基因名称 | DSE |
| Gene ID (NCBI) | 29940 |
| RRID | AB_2093286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Caprylic Acid-Ammonium Sulfate Precipitation |
| UNIPROT ID | Q9UL01 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DSE antibody 60041-1-Ig | Download protocol |
| IHC protocol for DSE antibody 60041-1-Ig | Download protocol |
| WB protocol for DSE antibody 60041-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Immunoglobulin G inhibits glucocorticoid-induced osteoporosis through occupation of FcγRI | ||
Inflamm Regen IFN-γ-STAT1-mediated CD8+ T-cell-neural stem cell cross talk controls astrogliogenesis after spinal cord injury |
