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验证数据展示

  • WB analysis using 13439-1-AP

    WB analysis using 13439-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13439-1-AP (DSCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13439-1-AP

    IHC staining of mouse brain using 13439-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13439-1-AP (DSCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 13439-1-AP

    IF Staining of mouse eye using 13439-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using DSCAM antibody (13439-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DSCAM Polyclonal antibody

DSCAM Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13439-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Cell adhesion molecule DSCAM, CHD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, mouse retina tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13439-1-AP targets DSCAM in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4248

Product name: Recombinant human DSCAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1760-2012 aa of NM_001389

Sequence: IDIPRAQLLIEERDTMETIDDRSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGPTARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSSMVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTSSTPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIHLPPYLRMDFLLNRGGPGTSRDLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQREGAELGQAAKMSSSQESLLDSRGHLKGNNPYAKSYTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Down syndrome cell adhesion molecule
别名 Cell adhesion molecule DSCAM, CHD2
计算分子量 2012 aa, 222 kDa
观测分子量250-270 kDa
GenBank蛋白编号NM_001389
基因名称 DSCAM
Gene ID (NCBI) 1826
RRIDAB_3669162
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60469
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DSCAM antibody 13439-1-APDownload protocol
IHC protocol for DSCAM antibody 13439-1-APDownload protocol
IF protocol for DSCAM antibody 13439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols