验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, SMMC-7721 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14743-1-AP targets DRG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | DRG2 fusion protein Ag6433 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | developmentally regulated GTP binding protein 2 |
| 别名 | Translation factor GTPase DRG2, TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 41-45 kDa |
| GenBank蛋白编号 | BC000493 |
| 基因名称 | DRG2 |
| Gene ID (NCBI) | 1819 |
| RRID | AB_2230800 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55039 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IHC protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IF protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IP protocol for DRG2 antibody 14743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. | ||
Int J Mol Sci DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial Dysfunction.
| ||
Oxid Med Cell Longev DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts
| ||
FASEB J Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain. | ||
Mol Cell Endocrinol RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation. | ||
Biochem Biophys Res Commun Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1.
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