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验证数据展示

  • WB analysis using 11020-1-AP

    WB analysis using 11020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11020-1-AP

    IHC staining of human pancreas cancer using 11020-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11020-1-AP (DRAP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DRAP1 antibody included
  • IF Staining of HepG2 using 11020-1-AP

    IF Staining of HepG2 using 11020-1-AP

    Immunofluorescent analysis of HepG2 cells, using DRAP1 antibody 11020-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

DRAP1 Polyclonal antibody

DRAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11020-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NC2-alpha, NC2 alpha, Dr1-associated protein 1, Dr1-associated corepressor, Dr1 associated protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, human heart tissue, human brain tissue, mouse kidney tissue, rat testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DRAP1 Ready-To-Use IHC Kit. DRAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11020-1-AP targets DRAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1470

Product name: Recombinant human DRAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC010025

Sequence: MPSKKKKYNARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFLKDLVASVPDMQGDGEDNHMDGDKGARRGRKPGSGGRKNGGMGTKSKDKKLSGTDSEQEDESEDTDTDGEEETSQPPPQASHPSAHFQSPPTPFLPFASTLPLPPAPPGPSAPDEEDEEDYDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DR1-associated protein 1 (negative cofactor 2 alpha)
别名 NC2-alpha, NC2 alpha, Dr1-associated protein 1, Dr1-associated corepressor, Dr1 associated protein 1
计算分子量 22 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC010025
基因名称 DRAP1
Gene ID (NCBI) 10589
RRIDAB_2277501
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14919
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DRAP1 antibody 11020-1-APDownload protocol
IHC protocol for DRAP1 antibody 11020-1-APDownload protocol
IF protocol for DRAP1 antibody 11020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols