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DRAP1 Polyclonal antibody

DRAP1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11020-1-AP

Print datasheet

Synonyms

Dr1 associated corepressor, Dr1 associated protein 1, DRAP1, NC2 alpha, Negative co factor 2 alpha

验证数据展示

  • WB analysis using 11020-1-AP

    WB analysis using 11020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11020-1-AP (DRAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11020-1-AP

    IHC staining of human pancreas cancer using 11020-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11020-1-AP (DRAP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DRAP1 antibody included
  • IF Staining of HepG2 using 11020-1-AP

    IF Staining of HepG2 using 11020-1-AP

    Immunofluorescent analysis of HepG2 cells, using DRAP1 antibody 11020-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse testis tissue, human heart tissue, human brain tissue, mouse kidney tissue, rat testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DRAP1 Ready-To-Use IHC Kit. DRAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11020-1-AP targets DRAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen DRAP1 fusion protein Ag1470 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DR1-associated protein 1 (negative cofactor 2 alpha)
Synonyms Dr1 associated corepressor, Dr1 associated protein 1, DRAP1, NC2 alpha, Negative co factor 2 alpha
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC010025
Gene Symbol DRAP1
Gene ID (NCBI) 10589
RRIDAB_2277501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14919
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DRAP1 antibody 11020-1-APDownload protocol
IHC protocol for DRAP1 antibody 11020-1-APDownload protocol
IF protocol for DRAP1 antibody 11020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols