验证数据展示
经过测试的应用
Positive IHC detected in | human stomach cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
16281-1-AP targets DPY30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | DPY30 fusion protein Ag9370 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dpy-30 homolog (C. elegans) |
别名 | dpy 30 homolog (C. elegans), Dpy 30 like protein, Dpy 30L, DPY30, HDPY 30, Protein dpy 30 homolog, Saf19 |
计算分子量 | 99 aa, 11 kDa |
GenBank蛋白编号 | BC015970 |
基因名称 | DPY30 |
Gene ID (NCBI) | 84661 |
RRID | AB_2918044 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9C005 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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IHC protocol for DPY30 antibody 16281-1-AP | Download protocol |
IF protocol for DPY30 antibody 16281-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |