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验证数据展示

  • WB analysis of rat skin using 14777-1-AP

    WB analysis of rat skin using 14777-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 14777-1-AP (Dermatopontin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Dermatopontin Polyclonal antibody

Dermatopontin Polyclonal Antibody for WB, ELISA
Cat No. 14777-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

DPT, Tyrosine-rich acidic matrix protein, TRAMP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat skin tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14777-1-AP targets Dermatopontin in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag6200

Product name: Recombinant human DPT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-201 aa of BC033736

Sequence: MDLSLLWVLLPLVTMAWGQYGDYGYPYQQYHDYSDDGWVNLNRQGFSYQCPQGQVIVAVRSIFSKKEGSDRQWNYACMPTPQSLGEPTECWWEEINRAGMEWYQTCSNNGLVAGFQSRYFESVLDREWQFYCCRYSKRCPYSCWLTIEYPGHYGEEMDMISYNYDYYIRGATTTFSAVERDRQWKFIMCRMTEYDCEFANV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dermatopontin
别名 DPT, Tyrosine-rich acidic matrix protein, TRAMP
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC033736
基因名称 DPT/TRAMP
Gene ID (NCBI) 1805
RRIDAB_2878078
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07507
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly and wound healing.

实验方案

Product Specific Protocols
WB protocol for Dermatopontin antibody 14777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols