验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue, rat skin tissue, human skeletal muscle tissue |
| Positive IHC detected in | human pancreas tissue, rat testis tissue, human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10537-1-AP targets Dermatopontin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0801 Product name: Recombinant human DPT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC033736 Sequence: MDLSLLWVLLPLVTMAWGQYGDYGYPYQQYHDYSDDGWVNLNRQGFSYQCPQGQVIVAVRSIFSKKEGSDRQWNYACMPTPQSLGEPTECWWEEINRAGMEWYQTCSNNGLVAGFQSRYFESVLDREWQFYCCRYSKRCPYSCWLTIEYPGHYGEEMDMISYNYDYYIRGATTTFSAVERDRQWKFIMCRMTEYDCEFANV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dermatopontin |
| 别名 | Dermatopontin, DPT, TRAMP |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC033736 |
| 基因名称 | DPT/TRAMP |
| Gene ID (NCBI) | 1805 |
| RRID | AB_2230721 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q07507 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the growth inhibition activity of TGF-beta1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Dermatopontin antibody 10537-1-AP | Download protocol |
| WB protocol for Dermatopontin antibody 10537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
Biomark Res Functional heterogeneity of cancer-associated fibroblasts with distinct neoadjuvant immunotherapy plus chemotherapy response in esophageal squamous cell carcinoma | ||
Neuro Oncol Single-cell transcriptomes reveal the heterogeneity and microenvironment of vestibular schwannoma | ||
Cardiovasc Res Regulation of Ptbp1-controlled alternative splicing of pyruvate kinase muscle by Liver kinase b1 governs vascular smooth muscle cell plasticity in vivo | ||
Cell Death Dis DNMT3a-dermatopontin axis suppresses breast cancer malignancy via inactivating YAP | ||
Int J Oncol NRG1 regulates redox homeostasis via NRF2 in papillary thyroid cancer.
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