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验证数据展示

  • WB analysis using 85159-3-RR (same clone as 85159-3-PBS)

    WB analysis using 85159-3-RR (same clone as 85159-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85159-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85159-3-RR (same clone as 85159-3-PBS)

    IHC staining of mouse liver using 85159-3-RR (same clone as 85159-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85159-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 85159-3-RR (same clone as 85159-3-PBS)

    IHC staining of mouse kidney using 85159-3-RR (same clone as 85159-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85159-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01879-2

    Cytometric bead array standard curve of MP01879-2

    Cytometric bead array standard curve of MP01879-2, MOUSE DPP4/CD26 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85159-3-PBS. Detection antibody: 85159-2-PBS. Standard: Eg1384. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01879-1

    Cytometric bead array standard curve of MP01879-1

    Cytometric bead array standard curve of MP01879-1, MOUSE DPP4/CD26 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85159-3-PBS. Detection antibody: 85159-1-PBS. Standard: Eg1384. Range: 0.781-100 ng/mL.

Mouse DPP4/CD26 Recombinant antibody, PBS Only (Capture)

DPP4/CD26 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 85159-3-PBS

产品说明书

CloneNo. 242823E10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

242823E10, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85159-3-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP01879-1: 85159-3-PBS capture and 85159-1-PBS detection (validated in Cytometric bead array)

MP01879-2: 85159-3-PBS capture and 85159-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 DPP4/CD26 fusion protein Eg1384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dipeptidylpeptidase 4
别名 242823E10, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form
计算分子量87 kDa
观测分子量110 kDa
GenBank蛋白编号NM_010074.3
基因名称 Dpp4
Gene ID (NCBI) 13482
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28843
储存缓冲液 PBS only{{ptg:BufferTemp}}7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).