验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33565-1-AP targets DPPA3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34680 Product name: Recombinant human DPPA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC160067 Sequence: MDPSQFNPTYIPGSPQMLTEENSRDDSGASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDEWLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESRPFKCPCSFCVSNGWDPSENARIGNQDTKPLQP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | developmental pluripotency associated 3 |
| 别名 | Developmental pluripotency-associated protein 3, STELLAR, Stella-related protein |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC160067 |
| 基因名称 | DPPA3 |
| Gene ID (NCBI) | 359787 |
| RRID | AB_3743011 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6W0C5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPPA3 (developmental pluripotency related 3), also known as STELLAR, which is a protein which plays a key role in embryonic development and epigenetic reprogramming, and is specifically expressed in embryonic stem cells and germ-line cells. In mice, DPPA3, as a maternal factor, plays a role in the pre-implantation development stage, participating in transcription inhibition, cell division and cell pluripotency maintenance. The predicted molecular weight is 17 kDa and the observed molecular weight is 23 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPPA3 antibody 33565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
