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验证数据展示

  • WB analysis using 28143-1-AP

    WB analysis using 28143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28143-1-AP (DPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 28143-1-AP

    IP experiment of mouse brain using 28143-1-AP

    IP result of anti-DPP6 (IP:28143-1-AP, 4ug; Detection:28143-1-AP 1:4000) with mouse brain tissue lysate 1840 ug.

  • IF Staining of SH-SY5Y using 28143-1-AP

    IF Staining of SH-SY5Y using 28143-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DPP6 antibody (28143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DPP6 Polyclonal antibody

DPP6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 28143-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28143-1-AP targets DPP6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27338

Product name: Recombinant human DPP6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 377-596 aa of BC150304

Sequence: KDGRKFFFIRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITSGDWDVTKILAYDEKGNKIYFLSTEDLPRRRQLYSANTVGNFNRQCLSCDLVENCTYFSASFSHSMDFFLLKCEGPGVPMVTVHNTTDKKKMFDLETNEHVKKAINDRQMPKVEYRDIEIDDYNLPMQILKPATFTDTTHYPLLLVVDGTPGSQSVAEKFEVSWETVMVSSHGAVVVKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 6
别名 DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein
观测分子量 98-101 kDa
GenBank蛋白编号BC150304
基因名称 DPP6
Gene ID (NCBI) 1804
RRIDAB_3669647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42658
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP6 (Dipeptidyl aminopeptidase-like protein 6) is also named as DPPX and Dipeptidyl peptidase VI (DPP VI). DPP6 is a type II transmembrane protein, and DPP6 is a multifunctional protein with considerable impact on brain development and function (PMID: 35395074). In breast cancer tissues, DPP6 has low expression at the transcription and protein levels, and DPP6 may serve as a tumour suppressor in tumour development (PMID: 36531033).

实验方案

Product Specific Protocols
IF protocol for DPP6 antibody 28143-1-APDownload protocol
IP protocol for DPP6 antibody 28143-1-APDownload protocol
WB protocol for DPP6 antibody 28143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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