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验证数据展示

  • WB analysis using 12553-1-AP

    WB analysis using 12553-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12553-1-AP (DPP10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12553-1-AP

    WB analysis using 12553-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12553-1-AP (DPP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

DPP10 Polyclonal antibody

DPP10 Polyclonal Antibody for WB, ELISA
Cat No. 12553-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2, DPL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue, pig brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12553-1-AP targets DPP10 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag3231

Product name: Recombinant human DPP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 448-796 aa of BC030832

Sequence: RGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFLLFCEGPRVPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQYALLLIMDEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITAVKFLLKLPYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEESTYQAASVLHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLYSTILKFFSDCLKEEISVLPQEPEEDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 10
别名 dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2, DPL2
计算分子量 796 aa, 91 kDa
观测分子量 91 kDa
GenBank蛋白编号BC030832
基因名称 DPP10
Gene ID (NCBI) 57628
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N608
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP10 (dipeptidyl aminopeptidase-like protein 10) is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels (PMID: 30638748). DPP10 is prominently expressed in the brain, notably present in neuronal populations (PMID: 15671030).

实验方案

Product Specific Protocols
WB protocol for DPP10 antibody 12553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols