验证数据展示
经过测试的应用
| Positive WB detected in | A-204 cells, HL-60 cells, Raji cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33922-1-AP targets DPH5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38953 Product name: Recombinant human DPH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-285 aa of NM_001077394.1 Sequence: WRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DPH5 homolog (S. cerevisiae) |
| 别名 | AD-018, CGI-30, Diphthamide biosynthesis methyltransferase, Diphthine methyl ester synthase, EC:2.1.1.314 |
| 计算分子量 | 32kDa，285aa |
| 观测分子量 | 26-36 kDa |
| GenBank蛋白编号 | NM_001077394.1 |
| 基因名称 | DPH5 |
| Gene ID (NCBI) | 51611 |
| RRID | AB_3743111 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H2P9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The research background of DPH5 mainly revolves around its key role in diphthamide amide biosynthesis pathway. Diphtheria amide is a highly conserved post-translational modification of eukaryotic translation elongation factor eEF2, which is very important for maintaining translation accuracy and protein synthesis efficiency. Due to modification, the molecular weight is 26-36 kDa. There are also some isoform and ubiquitination modifications.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPH5 antibody 33922-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
