DPH2 Polyclonal antibody
DPH2 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14866-1-AP targets DPH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB,ELISA |
| Tested Reactivity | human, mouse, rat |
| Immunogen | DPH2 fusion protein Ag6678 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DPH2 homolog (S. cerevisiae) |
| Synonyms | |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC001389 |
| Gene Symbol | DPH2 |
| Gene ID (NCBI) | 1802 |
| RRID | AB_2093254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPH2 antibody 14866-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |