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验证数据展示

  • WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

    WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

    WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

    IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

    IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

    IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

    IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

DPH2 Polyclonal antibody, PBS Only

DPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12367-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12367-1-PBS targets DPH2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3042

Product name: Recombinant human DPH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 190-489 aa of BC016956

Sequence: SPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DPH2 homolog (S. cerevisiae)
别名
计算分子量 489 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC016956
基因名称 DPH2
Gene ID (NCBI) 1802
RRIDAB_2093256
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQC3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

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