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DPF1 Polyclonal antibody

DPF1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21769-1-AP

Print datasheet

Synonyms

BAF45B, BRG1 associated factor 45B, DPF1, NEUD4, neuro d4, Zinc finger protein neuro d4

验证数据展示

  • WB analysis using 21769-1-AP

    WB analysis using 21769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21769-1-AP (DPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21769-1-AP

    IF Staining of MCF-7 using 21769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DPF1 antibody (21769-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, mouse cerebellum tissue
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy DPF1 Ready-To-Use IHC Kit. DPF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21769-1-AP targets DPF1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen DPF1 fusion protein Ag16409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name D4, zinc and double PHD fingers family 1
Synonyms BAF45B, BRG1 associated factor 45B, DPF1, NEUD4, neuro d4, Zinc finger protein neuro d4
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight38-47 kDa
GenBank Accession NumberBC125153
Gene Symbol DPF1
Gene ID (NCBI) 8193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92782
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPF1 antibody 21769-1-APDownload protocol
IF protocol for DPF1 antibody 21769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols