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验证数据展示

  • WB analysis using 31420-1-AP

    WB analysis using 31420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31420-1-AP (DPCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31420-1-AP

    IHC staining of human stomach cancer using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 31420-1-AP

    IHC staining of mouse stomach using 31420-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31420-1-AP (DPCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DPCR1 Polyclonal antibody

DPCR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31420-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PBLT, MUCL3, C6orf37

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBGC-823 cells, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31420-1-AP targets DPCR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33318

Product name: Recombinant human DPCR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 70-448 aa of NM_080870

Sequence: DHIVLHSGQRPPELPKSTEIHEQKRHCNTTRHSKPTDKPTGNSKTIDHKSSTDNHEAPPTSEENSSNQGKDPMIRNQRSVDPADSTTTHKESAGKKHITPAPKSKINCRKSTTGKSTVTRKSDKTGRPLEKSMSTLDKTSTSSHKTTTSFHNSGNSQTKQKSTSFPEKITAASKTTYKTTGTPEESEKTEDSRTTVASDKLLTKTTKNIQETISANELTQSLAEPTEHGGRTANENNTPSPAEPTENRERTANENKKTICTKGKNTPVPEKPTENLGNTTLTTETIKAPVKSTENPEKTAAVTKTIKPSVKVTGDKSLTTTSSHLNKTEVTHQVPTGSFTLITSRTKLSSITSEATGNESHPYLNKDGSQKGIHAGQMGENDSFPAWA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diffuse panbronchiolitis critical region 1
别名 PBLT, MUCL3, C6orf37
计算分子量56 kDa
观测分子量56 kDa
GenBank蛋白编号NM_080870
基因名称 DPCR1
Gene ID (NCBI) 135656
RRIDAB_3669973
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ3MIW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diffuse panbronchiolitis critical region 1 (DPCR1) is located in the major histocompatibility complex (MHC) class I. DPCR1 is downregulated in invasive pituitary adenoma compared with that in non-invasive tumors, but upregulated in the precursor of gastric carcinogenesis (PMID: 29242154).

实验方案

Product Specific Protocols
IHC protocol for DPCR1 antibody 31420-1-APDownload protocol
WB protocol for DPCR1 antibody 31420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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