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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    IF Staining of LNCaP using 68151-1-Ig (same clone as 68151-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

DOHH Monoclonal antibody, PBS Only

DOHH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68151-1-PBS

产品说明书

CloneNo. 1H5H7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68151-1-PBS targets DOHH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DOHH fusion protein Ag13041 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine hydroxylase/monooxygenase
别名 HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7
计算分子量 33 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC002817
基因名称 DOHH
Gene ID (NCBI) 83475
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BU89
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).