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验证数据展示

  • WB analysis using 83270-2-RR (same clone as 83270-2-PBS)

    WB analysis using 83270-2-RR (same clone as 83270-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83270-2-RR (DOCK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 83270-2-RR (same clone as 83270-2-PBS)

    IHC staining of mouse kidney using 83270-2-RR (same clone as 83270-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83270-2-RR (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DOCK8 antibody included
  • IF Staining of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

    IF Staining of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DOCK8 antibody (83270-2-RR, Clone: 230441A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

    FC experiment of THP-1 using 83270-2-RR (same clone as 83270-2-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83270-2-RR (same clone as 83270-2-PBS)

    FC experiment of HeLa using 83270-2-RR (same clone as 83270-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83270-2-PBS in a different storage buffer formulation.

DOCK8 Recombinant monoclonal antibody, PBS Only

DOCK8 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83270-2-PBS

产品说明书

CloneNo. 230441A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

230441A2, dedicator of cytokinesis 8, MRD2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DOCK8 Ready-To-Use IHC Kit. DOCK8 primary antibody included.

产品信息

83270-2-PBS targets DOCK8 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34585

Product name: Recombinant human DOCK8 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 257-606 aa of BC019102

Sequence: EVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPAITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPYIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dedicator of cytokinesis 8
别名 230441A2, dedicator of cytokinesis 8, MRD2
计算分子量 2031 aa, 231 kDa
观测分子量 239 kDa
GenBank蛋白编号BC019102
基因名称 DOCK8
Gene ID (NCBI) 81704
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NF50
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

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