验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
20683-1-AP targets DOCK3; MOCA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dedicator of cytokinesis 3 |
| 别名 | dedicator of cytokinesis 3, DOCK3, DOCK3; MOCA, KIAA0299, MOCA, Modifier of cell adhesion, PBP, Presenilin binding protein |
| 计算分子量 | 233 kDa |
| 观测分子量 | 233 kDa |
| GenBank蛋白编号 | NM_004947 |
| 基因名称 | DOCK3 |
| Gene ID (NCBI) | 1795 |
| RRID | AB_10696182 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZD9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK3; MOCA antibody 20683-1-AP | Download protocol |
| IHC protocol for DOCK3; MOCA antibody 20683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetes Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing. | ||
Int J Biochem Cell Biol Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer. |
