• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DOC2A Polyclonal antibody

DOC2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20575-1-AP

Print datasheet

Synonyms

Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha

验证数据展示

  • WB analysis using 20575-1-AP

    WB analysis using 20575-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20575-1-AP (DOC2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20575-1-AP

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20575-1-AP

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20575-1-AP targets DOC2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name double C2-like domains, alpha
Synonyms Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha
Calculated Molecular Weight 44 kDa
Observed Molecular Weight18-44 kDa
GenBank Accession NumberNM_003586
Gene Symbol DOC2A
Gene ID (NCBI) 8448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14183
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOC2, Double C2-like domain-containing protein alpha, is involved in Ca(2+)-dependent neurotransmitter release. DOC2A and DOC2B are sensors for neuronal activity with unique calcium-dependent and kinetic properties (PMID: 16515538). DOC2A is mainly expressed in brain and also expressed in testis (PMID: 7826360).

实验方案

Product Specific Protocols
WB protocol for DOC2A antibody 20575-1-APDownload protocol
IHC protocol for DOC2A antibody 20575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols