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验证数据展示

  • WB analysis of HeLa cells using 11637-1-AP

    WB analysis of HeLa cells using 11637-1-AP

    HeLa cell were subjected to SDS PAGE followed by western blot with 11637-1-AP (DNTTIP1 Antibody) at dilution of 1:500.

  • IP experiment of HeLa using 11637-1-AP

    IP experiment of HeLa using 11637-1-AP

    IP result of anti-DNTTIP1 (IP:11637-1-AP, 4ug; Detection:11637-1-AP 1:300) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 11637-1-AP

    IF Staining of HeLa using 11637-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNTTIP1 antibody (11637-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

DNTTIP1 Polyclonal antibody

DNTTIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11637-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

Terminal deoxynucleotidyltransferase-interacting factor 1, TdT-interacting factor 1, TdIF1, DNTTIP 1, Deoxynucleotidyltransferase terminal-interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11637-1-AP targets DNTTIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2213

Product name: Recombinant human DNTTIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC024290

Sequence: MGATGDAEQPRGPSGAERGGLELGDAGAAGQLVLTNPWNIMIKHRQVQRRGRRSQMTTSFTDPAISMDLLRAVLQPSINEEIQTVFNKYMKFFQKAALNVRDNVGEEVDAEQLIQEACRSCLEQAKLLFSDGEKVIPRLTHELPGIKRGRQAEEECAHRGSPLPKKRKGRPPGHILSSDRAAAGMVWKPKSCEPIRREGPKWDPARLNESTTFVLGSRANKALGMGGTRGRIYIKHPHLFKYAADPQDKHWLAEQHHMRATGGKMAYLLIEEDIRDLAASDDYRGCLDLKLEELKSFVLPSWMVEKMRKYMETLRTENEHRAVEAPPQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxynucleotidyltransferase, terminal, interacting protein 1
别名 Terminal deoxynucleotidyltransferase-interacting factor 1, TdT-interacting factor 1, TdIF1, DNTTIP 1, Deoxynucleotidyltransferase terminal-interacting protein 1
计算分子量 329 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC024290
基因名称 DNTTIP1
Gene ID (NCBI) 116092
RRIDAB_2877787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H147
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNTTIP1, also named as Deoxynucleotidyltransferase terminal-interacting protein 1, is a 329 amino acid protein ,which contains a Nuclear localization signal and localizes in the nuclear. DNTTIP1 also acts as a transcriptional regulator, which binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription.

实验方案

Product Specific Protocols
IF protocol for DNTTIP1 antibody 11637-1-APDownload protocol
IP protocol for DNTTIP1 antibody 11637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols