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验证数据展示

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of Raji using 17382-1-AP

    IP experiment of Raji using 17382-1-AP

    IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

  • FC experiment of Jurkat using 17382-1-AP

    FC experiment of Jurkat using 17382-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DNTT,TDT Polyclonal antibody (17382-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for IHC, FC (Intra), IP, ELISA
Cat No. 17382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), IP, ELISA

别名

DNTT, Terminal Deoxynucleotidyl Transferase, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17382-1-AP targets DNTT,TDT in IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11235

Product name: Recombinant human DNTT,TDT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 162-509 aa of BC012920

Sequence: NNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxynucleotidyltransferase, terminal
别名 DNTT, Terminal Deoxynucleotidyl Transferase, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase
计算分子量 509 aa, 58 kDa
GenBank蛋白编号BC012920
基因名称 DNTT
Gene ID (NCBI) 1791
RRIDAB_2878399
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04053
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
IP protocol for DNTT,TDT antibody 17382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols