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验证数据展示

  • WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

    WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

    IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 26971-1-AP (same clone as 26971-1-PBS)

    IP experiment of K-562 using 26971-1-AP (same clone as 26971-1-PBS)

    IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DNMT3B Polyclonal antibody, PBS Only

DNMT3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 26971-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26971-1-PBS targets DNMT3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25117

Product name: Recombinant human DNMT3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001207055

Sequence: MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA (cytosine-5-)-methyltransferase 3 beta
别名 ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B
计算分子量 95 kDa
观测分子量 85-96 kDa
GenBank蛋白编号NM_001207055
基因名称 DNMT3B
Gene ID (NCBI) 1789
RRIDAB_2880705
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBC3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

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