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验证数据展示

  • WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82678-2-RR

    Affinity and Kinetic Characterization of 82678-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82678-2-RR against Human DNMT1 were performed. The affinity constant is below 1 pM.

DNMT1 Recombinant monoclonal antibody, PBS Only

DNMT1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82678-2-PBS

产品说明书

CloneNo. 240302E2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), Indirect ELISA

别名

240302E2, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82678-2-PBS targets DNMT1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29479

Product name: Recombinant human DNMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 300-500 aa of BC126227

Sequence: KKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA (cytosine-5-)-methyltransferase 1
别名 240302E2, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1
计算分子量 1632 aa, 185 kDa
GenBank蛋白编号BC126227
基因名称 DNMT1
Gene ID (NCBI) 1786
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26358
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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