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  • KD/KO Validated

DNMT1 Polyclonal antibody

DNMT1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (5)


IF/ICC, IHC, IP, WB, ELISA and More (2)



Cat No : 24206-1-AP

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AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, MCMT


Positive WB detected inHeLa cells, C6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells


Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


24206-1-AP targets DNMT1 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP, ChIP
Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, zebrafish, pig
Immunogen DNMT1 fusion protein Ag19116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA (cytosine-5-)-methyltransferase 1
Synonyms AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, MCMT
Calculated Molecular Weight 1632 aa, 185 kDa
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)


Product Specific Protocols
WB protocol for DNMT1 antibody 24206-1-APDownload protocol
IHC protocol for DNMT1 antibody 24206-1-APDownload protocol
IF protocol for DNMT1 antibody 24206-1-APDownload protocol
IP protocol for DNMT1 antibody 24206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu


Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae

Authors - Fang Jiao

Bioact Mater

Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.

Authors - Jin Wang
  • KO Validated

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou

J Transl Med

The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy

Authors - Liangliang Zhao

Respir Res

Oxidative stress mediates the apoptosis and epigenetic modification of the Bcl-2 promoter via DNMT1 in a cigarette smoke-induced emphysema model.

Authors - Huihui Zeng