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验证数据展示

  • WB analysis using 31844-1-AP

    WB analysis using 31844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31844-1-AP (DNLZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 31844-1-AP

    IHC staining of rat heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 31844-1-AP

    IHC staining of mouse heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 31844-1-AP

    IF Staining of MCF-7 using 31844-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNLZ antibody (31844-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

DNLZ Polyclonal antibody

DNLZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31844-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

mtHsp70-escort protein, Hsp70-escort protein 1, HEP1, DNL-type zinc finger protein, C9orf151

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRT-4 cells
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31844-1-AP targets DNLZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37114

Product name: Recombinant human DNLZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-178 aa of BC146915

Sequence: MLRTALRGAPRLLSRVQPRAPCLRRLWGRGARPEVAGRRRAWAWGWRRSSSEQGPGPAAALGRVEAAHYQLVYTCKVCGTRSSKRISKLAYHQGVVIVTCPGCQNHHIIADNLGWFSDLNGKRNIEEILTARGEQVHRVAGEGALELVLEAAGAPTSTAAPEAGEDEGPPSPGKTEPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNL-type zinc finger
别名 mtHsp70-escort protein, Hsp70-escort protein 1, HEP1, DNL-type zinc finger protein, C9orf151
计算分子量19 kDa
观测分子量19 kDa
GenBank蛋白编号BC146915
基因名称 DNLZ
Gene ID (NCBI) 728489
RRIDAB_3670126
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5SXM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNL-type zinc finger (DNLZ, also known as HEP, ZIM17, TIMM15, and C9orf151) is a nuclear-encoded protein that is translocated to the mitochondria, thus it is located in mitochondrion and nucleoplasm (PMID: 22162012). It may function as a co-chaperone towards HSPA9/mortalin, which enhances HSPA9 solubility and stimulates HSPA9 catalytic activity (PMID: 18632665; 20392697; 21530495).

实验方案

Product Specific Protocols
IF protocol for DNLZ antibody 31844-1-APDownload protocol
IHC protocol for DNLZ antibody 31844-1-APDownload protocol
WB protocol for DNLZ antibody 31844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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