• Featured Product
  • KD/KO验证

DNASE2 Polyclonal antibody

DNASE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15934-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, MDA-MB-231 cells
Positive IP detected inU-87 MG cells, HeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15934-1-AP targets DNASE2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DNASE2 fusion protein Ag8781 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyribonuclease II, lysosomal
别名 DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha
计算分子量 360 aa, 40 kDa
观测分子量 32-40 kDa
GenBank蛋白编号BC010419
基因名称 DNASE2
Gene ID (NCBI) 1777
RRIDAB_2095546
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00115
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)

实验方案

Product Specific Protocols
WB protocol for DNASE2 antibody 15934-1-APDownload protocol
IHC protocol for DNASE2 antibody 15934-1-APDownload protocol
IF protocol for DNASE2 antibody 15934-1-APDownload protocol
IP protocol for DNASE2 antibody 15934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation

Authors - Biyao Wang
mouseWB

J Biol Chem

Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.

Authors - Xiaojuan Han
humanWB

Pharmacol Res

Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence

Authors - Danli Zhao
human,mouseWB

Mol Nutr Food Res

Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury

Authors - Lisi Li
mouseWB,IHC,IF

Transl Neurodegener

Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration

Authors - Ling-Jie Li
Loading...