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验证数据展示

  • WB analysis of mouse skeletal muscle using 13653-1-AP

    WB analysis of mouse skeletal muscle using 13653-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat skeletal muscle using 13653-1-AP

    WB analysis of rat skeletal muscle using 13653-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 13653-1-AP

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 13653-1-AP

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 13653-1-AP

    IF Staining of MCF-7 using 13653-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DNASE1L1 antibody 13653-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse skeletal muscle using 13653-1-AP

    IP experiment of mouse skeletal muscle using 13653-1-AP

    IP result of anti-DNASE1L1 (IP:13653-1-AP, 4ug; Detection:13653-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

  • Featured Product
  • KD/KO验证

DNASE1L1 Polyclonal antibody

DNASE1L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13653-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

DNase I-like 1, DNase I like 1, DNAS1L1, Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13653-1-AP targets DNASE1L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 DNASE1L1 fusion protein Ag4222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyribonuclease I-like 1
别名 DNase I-like 1, DNase I like 1, DNAS1L1, Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1
计算分子量 302 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC028092
基因名称 DNASE1L1
Gene ID (NCBI) 1774
RRIDAB_2230813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49184
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IHC protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IF protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IP protocol for DNASE1L1 antibody 13653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cells

New Findings: Hindlimb Unloading Causes Nucleocytoplasmic Ca2+ Overload and DNA Damage in Skeletal Muscle

Authors - Huajian Yang
View Article
ratWB

J Tradit Chin Med

Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

Authors - Zhu Bin
  • KD Validated
View Article