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验证数据展示

  • WB analysis using 85734-1-RR

    WB analysis using 85734-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85734-1-RR (DNALI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse fallopian tube using 85734-1-RR

    IHC staining of mouse fallopian tube using 85734-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue slide using 85734-1-RR (DNALI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85734-1-RR

    Affinity and Kinetic Characterization of 85734-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85734-1-RR against Human DNALI1 were performed. The affinity constant is below 1 pM.

DNALI1 Recombinant antibody

DNALI1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85734-1-RR

产品说明书

CloneNo. 243157E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hp28, P28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse fallopian tube tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNALI1 fusion protein Ag11652 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dynein, axonemal, light intermediate chain 1
别名 hp28, P28
计算分子量 258 aa, 30 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC046117
基因名称 DNALI1
Gene ID (NCBI) 7802
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

实验方案

Product Specific Protocols
WB protocol for DNALI1 antibody 85734-1-RRDownload protocol
IHC protocol for DNALI1 antibody 85734-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols