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验证数据展示

  • WB analysis using 85734-1-RR

    WB analysis using 85734-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85734-1-RR (DNALI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse fallopian tube using 85734-1-RR

    IHC staining of mouse fallopian tube using 85734-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue slide using 85734-1-RR (DNALI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85734-1-RR

    Affinity and Kinetic Characterization of 85734-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85734-1-RR against Human DNALI1 were performed. The affinity constant is below 1 pM.

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DNALI1 Recombinant monoclonal antibody

DNALI1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85734-1-RR

产品说明书

CloneNo. 243157E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hp28, P28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse fallopian tube tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11652

Product name: Recombinant human DNALI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-258 aa of BC046117

Sequence: MIPPADSLLKYDTPVLVSRNTEKRSPKARLLKVSPQQPGPSGSAPQPPKTKLPSTPCVPDPTKQAEEILNAILPPREWVEDTQLWIQQVSSTPSTRMDVVHLQEQLDLKLQQRQARETGICPVRRELYSQCFDELIREVTINCAERGLLLLRVRDEIRMTIAAYQTLYESSVAFGMRKALQAEQGKSDMERKIAELETEKRDLERQVNEQKAKCEATEKRESERRQVEEKKHNEEIQFLKRTNQQLKAQLEGIIAPKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dynein, axonemal, light intermediate chain 1
别名 hp28, P28
计算分子量 258 aa, 30 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC046117
基因名称 DNALI1
Gene ID (NCBI) 7802
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

实验方案

Product Specific Protocols
WB protocol for DNALI1 antibody 85734-1-RRDownload protocol
IHC protocol for DNALI1 antibody 85734-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols