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验证数据展示

  • WB analysis of HeLa using 21941-1-AP

    WB analysis of HeLa using 21941-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 21941-1-AP

    WB analysis using 21941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21941-1-AP

    IP experiment of mouse brain using 21941-1-AP

    IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug.

  • IF Staining of HeLa using 21941-1-AP

    IF Staining of HeLa using 21941-1-AP

    Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 21941-1-AP

    IHC staining of mouse brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21941-1-AP

    IHC staining of mouse brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNAJC6/AUXILIN Polyclonal antibody

DNAJC6/AUXILIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21941-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Auxilin, DNAJC6, DJC6, EC:3.1.3.-, KIAA0473

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21941-1-AP targets DNAJC6/AUXILIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DNAJC6/AUXILIN fusion protein Ag16612 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 6
别名 Auxilin, DNAJC6, DJC6, EC:3.1.3.-, KIAA0473
计算分子量 970 aa, 106 kDa
观测分子量 100-110 kDa, 86 kDa
GenBank蛋白编号BC109279
基因名称 DNAJC6/AUXILIN
Gene ID (NCBI) 9829
RRIDAB_2878951
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

实验方案

Product Specific Protocols
WB protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IHC protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IF protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IP protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
View Article
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article