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验证数据展示

  • WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

    WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 12096-1-AP (same clone as 12096-1-PBS)

    IP experiment of mouse heart using 12096-1-AP (same clone as 12096-1-PBS)

    IP result of anti-DNAJC19 (IP:12096-1-AP, 4ug; Detection:12096-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12096-1-AP (same clone as 12096-1-PBS)

    IF Staining of HepG2 using 12096-1-AP (same clone as 12096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJC19 antibody (12096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DNAJC19 Polyclonal antibody, PBS Only

DNAJC19 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12096-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12096-1-PBS targets DNAJC19 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2739

Product name: Recombinant human DNAJC19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-116 aa of BC009702

Sequence: MASTVVAVGLTIAAAGFAGRYVLQAMKHMEPQVKQVFQSLPKSAFSGGYYRGGFEPKMTKREAALILGVSPTANKGKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 19
别名
计算分子量 116 aa, 13 kDa
观测分子量 13 kDa
GenBank蛋白编号BC009702
基因名称 DNAJC19
Gene ID (NCBI) 131118
RRIDAB_2094914
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DA6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)

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