验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31580-1-AP targets DNAJC17 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35992 Product name: Recombinant human DNAJC17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 69-194 aa of BC000048 Sequence: RAAYDKVRKAKKQAAERTQKLDEKRKKVKLDLEARERQAQAQESEEEEESRSTRTLEQEIERLREEGSRQLEEQQRLIREQIRQERDQRLRGKAENTEGQGTPKLKLKWKCKKEDESKGGYSKDVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily C, member 17 |
| 别名 | FLJ10634 |
| 计算分子量 | 304 aa, 35 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000048 |
| 基因名称 | DNAJC17 |
| Gene ID (NCBI) | 55192 |
| RRID | AB_3670044 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NVM6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DnaJ proteins, also known as Hsp40, are a set of highly conserved proteins typically acting as co-factors of Hsp70 molecular chaperones. Based on their domain architecture, DNAJ is classified into three different groups (A, B, and C). DNAJC17 belongs to group C, bearing the J domain at its N-terminal region and an RNA recognition motif (RRM) located at the protein C-terminu. DNAJC17 is a susceptibility gene for congenital hypothyroidism and demonstrated its wide expression in mice since early embryogenesis, in which it plays an essential role in pre-implantation stages (PMID: 29773831).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC17 antibody 31580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
