验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, BxPC-3 cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DNAJC12 Ready-To-Use IHC Kit. DNAJC12 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2989 Product name: Recombinant human DNAJC12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC017018 Sequence: MDAILNYRSEDTEDYYTLLGCDELSSVEQILAEFKVRALECHPDKHPENPKAVETFQKLQKAKEILTNEESRARYDHWRRSQMSMPFQQWEALNDSVKTSMHWVVRGKKDLMLEESDKTHTTKMENEECNEQRERKKEELASTAEKTEQKEPKPLEKSVSPQNSDSSGFADVNGWHLRFRWSKDAPSELLRKFRNYEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily C, member 12 |
| 别名 | DNAJC12, J domain containing protein 1, JDP1 |
| 计算分子量 | 198 aa, 23 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC017018 |
| 基因名称 | DNAJC12 |
| Gene ID (NCBI) | 56521 |
| RRID | AB_2246188 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKB3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| IHC protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Cell Stress Chaperones The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress. | ||
J Mol Cell Biol DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation
| ||
Ann Surg Oncol Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.
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