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DNAJB5 Polyclonal antibody

DNAJB5 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16453-1-AP

Print datasheet

Synonyms

DNAJB5, Heat shock protein cognate 40, Heat shock protein Hsp40 2, Heat shock protein Hsp40 3, Hsc40, KIAA1045

验证数据展示

  • WB analysis of rat heart using 16453-1-AP

    WB analysis of rat heart using 16453-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16453-1-AP

    IP experiment of HepG2 using 16453-1-AP

    IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16453-1-AP

    IF Staining of HepG2 using 16453-1-AP

    Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat heart tissue, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman heart tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16453-1-AP targets DNAJB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen DNAJB5 fusion protein Ag9341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 5
Synonyms DNAJB5, Heat shock protein cognate 40, Heat shock protein Hsp40 2, Heat shock protein Hsp40 3, Hsc40, KIAA1045
Calculated Molecular Weight 386 aa, 44 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC012115
Gene Symbol DNAJB5
Gene ID (NCBI) 25822
RRIDAB_2094724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75953
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.

实验方案

Product Specific Protocols
WB protocol for DNAJB5 antibody 16453-1-APDownload protocol
IHC protocol for DNAJB5 antibody 16453-1-APDownload protocol
IF protocol for DNAJB5 antibody 16453-1-APDownload protocol
IP protocol for DNAJB5 antibody 16453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols