验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3756 Product name: Recombinant human DNAJB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-337 aa of BC034721 Sequence: MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 4 |
| 别名 | Human liver DnaJ like protein, HSP40 homolog, HLJ1, Heat shock protein 40 homolog, DNAJW |
| 计算分子量 | 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC034721 |
| 基因名称 | DNAJB4 |
| Gene ID (NCBI) | 11080 |
| RRID | AB_2094422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UDY4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| IHC protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| IP protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| WB protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4 | ||
Int J Mol Sci Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.
| ||
Int J Clin Exp Pathol HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival. | ||
Elife HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages
| ||
Acta Neuropathol Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure
|
