验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, A431 cells, mouse skeletal muscle tissue, rat heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10838-1-AP targets DNAJB2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DNAJB2 fusion protein Ag1271 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 2 |
| 别名 | HSJ-1, HSJ1, HSJ 1, Heat shock protein J1, Heat shock 40 kDa protein 3 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36-40 kDa |
| GenBank蛋白编号 | BC011609 |
| 基因名称 | DNAJB2 |
| Gene ID (NCBI) | 3300 |
| RRID | AB_2277491 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB2 antibody 10838-1-AP | Download protocol |
| IHC protocol for DNAJB2 antibody 10838-1-AP | Download protocol |
| IP protocol for DNAJB2 antibody 10838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann Neurol A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation. | ||
Eur J Neurol DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening. | ||
Sci Rep PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |
