验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, mouse heart tissue, HepG2 cells, rat liver tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DNAJB11 Ready-To-Use IHC Kit. DNAJB11 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15484-1-AP targets DNAJB11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7832 Product name: Recombinant human DNAJB11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-358 aa of BC001144 Sequence: GRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQGY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 11 |
| 别名 | EDJ, ABBP-2, APOBEC1-binding protein 2, DNAJB 11, Endoplasmic reticulum DNA J domain-containing protein 3 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC001144 |
| 基因名称 | DNAJB11 |
| Gene ID (NCBI) | 51726 |
| RRID | AB_2094400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBS4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| IHC protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| IP protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| WB protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.
| ||
Nat Cell Biol A multichaperone condensate enhances protein folding in the endoplasmic reticulum | ||
Mol Cell Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network. | ||
Cell Mol Biol Lett Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway
| ||
Cell Death Dis Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.
|
