验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, HeLa cells, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse ovary tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11585 Product name: Recombinant human DNAI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 239-593 aa of BC039582 Sequence: GQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, axonemal, intermediate chain 2 |
| 别名 | CILD9, Dynein axonemal intermediate chain 2 |
| 计算分子量 | 593 aa, 67 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC039582 |
| 基因名称 | DNAI2 |
| Gene ID (NCBI) | 64446 |
| RRID | AB_2096670 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZS0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| IHC protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| IP protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| WB protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Production of transgenic mice by random recombination of targeted genes in female germline stem cells. | ||
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
Development CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility | ||
Exp Anim The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. | ||
Ann Hum Genet DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella. |
