验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human brain tissue, rat testis tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 5 publications below |
产品信息
12756-1-AP targets DNAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3473 Product name: Recombinant human DNAI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-699 aa of BC030583 Sequence: VGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAIYNLKKPHSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLKVEGSTTEVPEGLQLHQVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSWNPYHTKVFMSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKYEAICNQPVAAKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVREVKIKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, axonemal, intermediate chain 1 |
| 别名 | PCD, ICS1, Dynein axonemal intermediate chain 1, CILD1 |
| 计算分子量 | 699 aa, 79 kDa |
| 观测分子量 | 80-85 kDa |
| GenBank蛋白编号 | BC030583 |
| 基因名称 | DNAI1 |
| Gene ID (NCBI) | 27019 |
| RRID | AB_10643244 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UI46 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAI1 antibody 12756-1-AP | Download protocol |
| WB protocol for DNAI1 antibody 12756-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. | ||
Elife DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice | ||
Cytoskeleton (Hoboken) Axonemal Dynein Visualized in Primary Cilia via Expansion Microscopy | ||
J Hum Genet Novel variants in DNAH9 are present in two infertile patients with severe asthenospermia | ||
EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men |
