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验证数据展示

  • IHC staining of human colon using 18841-1-AP

    IHC staining of human colon using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 18841-1-AP

    IHC staining of human colon using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 18841-1-AP

    IF Staining of U2OS using 18841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 18841-1-AP (DNAH2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 18841-1-AP

    IF Staining of A549 using 18841-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DNAH2 antibody (18841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DNAH2 Polyclonal antibody

DNAH2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18841-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA and More (1)

别名

Ciliary dynein heavy chain 2, DNAHC2, DNHD3, Dynein axonemal heavy chain 2, Dynein heavy chain domain-containing protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

18841-1-AP targets DNAH2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 2
别名 Ciliary dynein heavy chain 2, DNAHC2, DNHD3, Dynein axonemal heavy chain 2, Dynein heavy chain domain-containing protein 3
计算分子量 508 kDa
GenBank蛋白编号NM_020877
基因名称 DNAH2
Gene ID (NCBI) 146754
RRIDAB_2878565
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P225
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAH2, also named as DNAHC2, DNHD3 and KIAA1503, belongs to the dynein heavy chain family. DNAH2 force generating protein of respiratory cilia and produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. DNAH2 involved in sperm motility; implicated in sperm flagellar assembly.

实验方案

Product Specific Protocols
IHC protocol for DNAH2 antibody 18841-1-APDownload protocol
IF protocol for DNAH2 antibody 18841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Andrology

A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans

Authors - Shengjia Shi
View Article
humanIF

Asian J Androl

A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility

Authors - Zheng Zhou
View Article