验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 8 publications below |
产品信息
24488-1-AP targets DNAH17 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20033 Product name: Recombinant human DNAH17 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 299-644 aa of BC112284 Sequence: MEQADFTMLPTFIAKVLDTICFIWATSEYYNTPARIIVILQEFCNQIIEMTRTFLSPEEVLKGLQGEIEEVLSGISLAVNVLKELYQTYDFCCVNMKLFFKDKEPVPWEFPSSLAFSRINSFFQRIQTIEELYKTAIEFLKLEKIELGGVRGNLLGSLVTRIYDEVFELVKVFADCKYDPLDPGDSNFDRDYADFEIKIQDLDRRLATIFCQGFDDCSCIKSSAKLLYMCGGLMERPLILAEVAPRYSVMLELFDAELDNAKILYDAQMAASEEGNIPLIHKNMPPVAGQLKWSLELQERLEVSMKHLKHVEHPVMSGAEAKLTYQKYDEMMELLRCHREKIYQQW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, axonemal, heavy chain 17 |
| 别名 | DNAH17, DNAHL1, DNEL2 |
| 计算分子量 | 4485 aa, 512 kDa |
| 观测分子量 | 500-512 kDa |
| GenBank蛋白编号 | BC112284 |
| 基因名称 | DNAH17 |
| Gene ID (NCBI) | 8632 |
| RRID | AB_2879568 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UFH2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNAH17 antibody 24488-1-AP | Download protocol |
| WB protocol for DNAH17 antibody 24488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice | ||
Clin Genet Loss-of-function mutation in DNAH8 induces asthenoteratospermia associated with multiple morphological abnormalities of the sperm flagella. | ||
Reprod Biomed Online A novel mutation in DNAH17 is present in a patient with multiple morphological abnormalities of the flagella. | ||
J Assist Reprod Genet The effect of a novel LRRC6 mutation on the flagellar ultrastructure in a primary ciliary dyskinesia patient. | ||
Ann Hum Genet DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella. | ||
Hum Mol Genet Population-based genetic analysis in infertile men reveals novel mutations of ADAD family members in patients with impaired spermatogenesis |
