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验证数据展示

  • IHC staining of mouse lung using 31281-1-AP

    IHC staining of mouse lung using 31281-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31281-1-AP (DNAH11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNAH11 Polyclonal antibody

DNAH11 Polyclonal Antibody for IHC, ELISA
Cat No. 31281-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

DNHBL, Ciliary dynein heavy chain 11, CILD7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31281-1-AP targets DNAH11 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DNAH11 fusion protein Ag34658 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 11
别名 DNHBL, Ciliary dynein heavy chain 11, CILD7
计算分子量520 kDa
GenBank蛋白编号NM_001277115
基因名称 DNAH11
Gene ID (NCBI) 8701
RRIDAB_3669928
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DT5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAH11 (dynein axonemal heavy chain 11) encodes a ciliary outer dynein arm (ODA) protein. Human DNAH11 is important for organ lateralization and likely also for the function of respiratory cilia. DNAH11 mutations are a common cause of PCD with normal ciliary ultrastructure and hyperkinetic ciliary beating (PMID: 26909801).

实验方案

Product Specific Protocols
IHC protocol for DNAH11 antibody 31281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols