验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23228-1-AP targets DNAAF2 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag19741 Product name: Recombinant human C14orf104 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 606-837 aa of BC013322 Sequence: LRFSAQDLVYSFFLQFAPENKLSTTEPVISISSNNAVIELAKSPESHGHWREWYYGVNNDSLELQECSNSDQLQGKEERVNEESHLTEKEYIEHCNTPTTDSDSSIAVKALQIDSFGLVTCFQQESLDVSQMILGKSQQPESKMQSEFIKEKSATCSNEEKDNLNESVITEEKETDGDHLSSLLNKTTVHNIPGFDSIKETNMQDGSVQVIKDHVTNCAFSFQNSLLYDLD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 104 |
| 别名 | C14orf104, CILD10, Dynein assembly factor 2, axonemal, Dynein Axonemal Assembly Factor 2, KTU |
| 计算分子量 | 837 aa, 91 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC013322 |
| 基因名称 | C14orf104 |
| Gene ID (NCBI) | 55172 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9NVR5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAAF2 (Dynein Axonemal Assembly Factor 2) is a cytoplasmic protein involved in the assembly and maturation of outer dynein arms (ODAs) in ciliary and flagellar axonemes. DNAAF2 cooperates with DNAAF1, DNAAF3, and other factors to mediate the transport of dynein precursors from the cytoplasm to the ciliary base, and promotes the proper folding and localization of dynein complexes at the axonemal assembly site. Mutations in the DNAAF2 gene cause primary ciliary dyskinesia (PCD), characterized by chronic respiratory tract infections, situs inversus, and male infertility, due to defective mucociliary clearance resulting from impaired ciliary motility.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for DNAAF2 antibody 23228-1-AP | Download protocol |
| WB protocol for DNAAF2 antibody 23228-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
